Device

Part:BBa_J24676:Design

Designed by: Annie Gao   Group: iGEM06_Brown   (2006-10-26)


pBAD to tetR coding region, lacI coding region, and mCherry reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3094
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Strength of RBS may affect the strength of output.


Source

Composite of other iGEM parts

References